Journal: Regenerative Biomaterials

Article Title: Engineering stem cell exosomes promotes the survival of multi-territory perforator flap in diabetes via regulating anti-inflammatory and angiogenesis

doi: 10.1093/rb/rbaf075

Figure Lengend Snippet: ( A ) TEM images of M-MS NPs. Scale bars: 500 nm, 50 nm. ( B ) DLS images of BMSC exosomes. Scale bars: 500 nm, 50 nm. ( C ) Western blot images of protein expression of CD9, CD63 and TSG101 from BMSCs and BMSCs exosomes. ( D ) Schematic diagram of M-MS@EXO NPs preparation. ( E ) TEM images of BMSCs exosomes and M-MS@EXO NPs. Scale bars: 100 nm. ( F ) Zeta potential patterns of MS, M-MS, M-MS@EXO NPs. ( G ) The particle size of MS, M-MS, M-MS@EXO NPs. ( H ) The cumulative release profile of metformin from M-MS@EXO NPs.

Article Snippet: The rat bone marrow mesenchymal stem cells (BMSCs) were extracted from the bone marrow cavity of 4-week-old Sprague Dawley rats.

Techniques: Western Blot, Expressing, Zeta Potential Analyzer

Journal: Bioactive Materials

Article Title: Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications

doi: 10.1016/j.bioactmat.2025.11.012

Figure Lengend Snippet: Evaluation of biocompatibility, adhesion and migration ability of both BMSCs and HUVECs on CoCrMo-Mg scaffold. (A) Biocompatibility of BMSCs: (a) CCK-8 assay; (b) Live/dead staining of BMSCs at day 3; (c) Quantitative statistics of cell proliferation. (B) Biocompatibility of HUVECs: (a) CCK-8 assay; (b) Live/dead staining of HUVECs at day 3; (c) Quantitative statistics of cell proliferation. (C) Cell adhesion of BMSCs: (a) FE-SEM and EDS images of BMSCs co-cultured with CoCrMo and CoCrMo-Mg scaffolds for 48 h; (b) Quantitative statistics of cell length; (c) Quantitative statistics of filopodia per cell. (D) Cell adhesion of HUVECs: (a) FE-SEM and EDS images of HUVECs; (b) Quantitative statistics of cell length; (c) Quantitative statistics of filopodia per cell. (E) The process of Transwell assay in BMSCs and HUVECs cultured with CoCrMo and CoCrMo-Mg scaffolds. (F) Crystalline violet staining of migrated cells. (G) Migration counts of BMSCs and HUVECs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs), and RAW264.7 murine macrophage cell line (RAW264.7) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Migration, CCK-8 Assay, Staining, Cell Culture, Transwell Assay

Journal: Bioactive Materials

Article Title: Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications

doi: 10.1016/j.bioactmat.2025.11.012

Figure Lengend Snippet: Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and HIF-1α in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs), and RAW264.7 murine macrophage cell line (RAW264.7) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, ALP Assay

Journal: Regenerative Biomaterials

Article Title: Protein conformational transition microenvironment in silk fibroin hydrogels: proliferation and chondrogenesis of encapsulated stem cells

doi: 10.1093/rb/rbaf102

Figure Lengend Snippet: Effects of the varied protein conformational transition microenvironments on the proliferation of stem cells within hydrogels. ( A ) Relative total cell viability changes during the corresponding conformational transition processes; ( B ) Related change ratios of the LU values from the data of (A); ( C ) LSCM photographs of the live/dead staining of the MSCs within typical hydrogels after 1 week cell culture. ‘Δ’: P > 0.05; ‘*’: 0.01 < P < 0.05; ‘**’: 0.001 < P < 0.01; ‘***’: P < 0.001. The calculated P values for evaluating the statistical difference between indicated groups in (A) are presented in .

Article Snippet: Passage 0 (P0) rat bone marrow MSCs were obtained from Shanghai Yuchun Biotechnology Co., Ltd. Assay Buffer and TRIzol reagent were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Ethanol, chloroform and isopropanol were obtained from Sinopharm Group Chemical Reagent Co., Ltd.

Techniques: Staining, Cell Culture